Zhang Hong-yan,Lü Xiao-yi,Jia Zhen-hong,Li Jiang-wei,Zhang Fu-chun.Porous silicon biosensor for detection of variable domain of heavy-chain of HCAb antibody[J].Optoelectronics Letters,2012,8(2):81-83 Porous silicon biosensor for detection of variable domain of heavy-chain of HCAb antibody Author Name Affiliation Zhang Hong-yan  School of Physical Science and Technology, Xinjiang University, Urumqi 830046, China  Lü Xiao-yi  Postdoctoral Station of Computer Science and Technology, Xinjiang University, Urumqi 830046, China  Jia Zhen-hong  College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China  Li Jiang-wei  Key Laboratory of Xinjiang Biological Resources and Gene Engineering, College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China  Zhang Fu-chun  Key Laboratory of Xinjiang Biological Resources and Gene Engineering, College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China  Abstract:       In this paper, we produce porous silicon (PSi) by electrochemical etching, and it is the first time to evaluate the performance of label-free porous silicon biosensor for detection of variable domain of heavy chain of heavy-chain antibody (VHH). The binding of hen egg white lysozyme (HEWL) and VHH causes a red shift in the reflection spectrum of the biosensor. The red shift is proportional to the VHH concentration in the range from 14 g·ml−1 to 30 g·ml−1 with a detection limit of 0.648 ng·ml−1. The research is useful for the development of label-free biosensor applied in the rapid and sensitive determination of small molecules. Hits: 4797 Download times: 751 This work has been supported by the National Natural Science Foundation of China (No.60968002 View Full Text    Download reader