Zhong Fu-ru,Ma Xiao-lin,Lü Xiao-yi,Jia Zhen-hong.Label-free quantitative detection using porous silicon as optical biosensor[J].Optoelectronics Letters,2012,8(4):314-317 Label-free quantitative detection using porous silicon as optical biosensor Author Name Affiliation Zhong Fu-ru  School of Chemistry and Chemical Engineering, Xinjiang University, Urumqi 830046, China  Ma Xiao-lin  College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China  Lü Xiao-yi  College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China  Jia Zhen-hong  College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China  Abstract:       The single-layer porous silicon is prepared by electrochemistry etching method, which is used as an immunosensor for determining recombinant mouse zona pellucida 3 fusion protein (r-mZP3) by Raman spectroscopy analysis at room temperature. The molecule binding increases the effective optical thickness (EOT), and thus the Raman spectrum intensity decreases. The concentration and variation of Raman intensity show a good linear quantitative relation. The excellent sensing performance could open the way to a new family of optical sensors for biological standardization. Hits: 4148 Download times: 441 This work has been supported by the National Natural Science Foundation of China (No.60968002), and the China Postdoctoral Science Foundation (No.2011M501501). View Full Text    Download reader