SU Lan,ZHANG Ping,ZHENG Da-wei,WANG Yang-jun-qi,ZHONG Ru-gang.Rapid detection of Escherichia coli and Salmonella typhimurium by surface-enhanced Raman scattering[J].Optoelectronics Letters,2015,11(2):157-160 |
Rapid detection of Escherichia coli and Salmonella typhimurium by surface-enhanced Raman scattering |
Author Name | Affiliation | SU Lan | College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China | ZHANG Ping | College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China | ZHENG Da-wei | College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China | WANG Yang-jun-qi | College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China | ZHONG Ru-gang | College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China |
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Abstract: |
In this paper, the surface-enhanced Raman scattering (SERS) is used as an analytical tool for the detection and identification of pathogenic bacteria of Escherichia coli (E. coli) and Salmonella typhimurium (S. typhimurium). Compared with normal Raman signal, the intensity of SERS signal is greatly enhanced. After processing all SERS data, the obvious differences between the SERS spectra of two species are determined. And applying the chemometric tools of principal component analysis and hierarchical cluster analysis (PCA-HCA), the SERS spectra of two species are distinguished more accurately. The results indicate that SERS analysis can provide a rapid and sensitive method for the detection of pathogenic bacteria. |
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This work has been supported by the National Natural Science Foundation of China (No.21107005), and the Ph.D. Programs Foundation of Ministry of China (No.3C015001201201). |
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