| WU Ji-xuan,WANG Qian,SONG Bin-bin,LIU Bo,ZHANG Hao,ZHANG Cheng,DUAN Shao-xiang,BAI Hua.Fiber-coupler-based microfluidic system for trapping of DNA biomolecules[J].Optoelectronics Letters,2019,15(6):476- |
| Fiber-coupler-based microfluidic system for trapping of DNA biomolecules |
| Author Name | Affiliation | | WU Ji-xuan | Tianjin Key Laboratory of Optoelectronic Detection Technology and Systems, Tianjin Key Laboratory of Engineering Technologies for Cell Phamaceutica, College of Electronic and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China | | WANG Qian | Tianjin Key Laboratory of Optoelectronic Detection Technology and Systems, Tianjin Key Laboratory of Engineering Technologies for Cell Phamaceutica, College of Electronic and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China | | SONG Bin-bin | Key Laboratory of Computer Vision and Systems, Ministry of Education, School of Computer Science and Engineering, Tianjin University of Technology, Tianjin 300384, China | | LIU Bo | Key Laboratory of Optoelectronic Sensor and Sensing Network Technology, College of Electronic Information and Optical Engineering, Nankai University, Tianjin 300350, China | | ZHANG Hao | Key Laboratory of Optoelectronic Sensor and Sensing Network Technology, College of Electronic Information and Optical Engineering, Nankai University, Tianjin 300350, China | | ZHANG Cheng | Tianjin Key Laboratory of Optoelectronic Detection Technology and Systems, Tianjin Key Laboratory of Engineering Technologies for Cell Phamaceutica, College of Electronic and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China | | DUAN Shao-xiang | Key Laboratory of Optoelectronic Sensor and Sensing Network Technology, College of Electronic Information and Optical Engineering, Nankai University, Tianjin 300350, China | | BAI Hua | Tianjin Key Laboratory of Optoelectronic Detection Technology and Systems, Tianjin Key Laboratory of Engineering Technologies for Cell Phamaceutica, College of Electronic and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China |
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| Abstract: |
| A miniature fiber-coupler-based microfluidic system is proposed for trapping of DNA biomolecules. The loop-shaped fiber-coupler is fabricated by using flame tapering technique and integrated in a microfluidic channel. Probe-DNA immobilized on the fiber-coupler surface enables specific recognition of target DNA sequences and effectively facilitates the trapping of target DNA molecules. The binding characteristics of biomolecules on the fiber-coupler surface have been theoretically analyzed and experimentally demonstrated. Experimental results indicate that the spectral response of the loop-shaped fiber coupler immobilized with probe DNA exhibits a red-shift with the trapping of the DNA biomolecules. The proposed microfluidic system possesses such desirable merits as simple structure, label-free method and high integration, which make it a promising candidate for applications in molecular biology and related bioengineering areas. |
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