In this paper,a digital holography system with short-coherence light source is used to record a series of holograms of a micro-object. The three dimensional reconstruction is completed by the least-square-polynomial-fitting with a series of two dimensional intensity images which are obtained through holographic reconstruction. This three dimensional reconstruction method can be used to carry out three-dimensional reconstruction of a micro-object with strong laser speckle noise, which can not be obtained from the conventional method.