| Ping Chen,Xing Li,Yan Wang,Hua Bai,Lie Lin.Distinguish on the viability of human umbilical cord mesenchymal stem cells using delayed luminescence[J].Optoelectronics Letters,2014,10(5):391-394 |
| Distinguish on the viability of human umbilical cord mesenchymal stem cells using delayed luminescence |
| Author Name | Affiliation | E-mail | | Ping Chen | Key Laboratory of Optical Information Science Technology, Ministry of Education of China, Institute of Modern Optics, Nankai University, Tianjin, 300071, China | | | Xing Li | Key Laboratory of Optical Information Science Technology, Ministry of Education of China, Institute of Modern Optics, Nankai University, Tianjin, 300071, China | | | Yan Wang | Key Laboratory of Optical Information Science Technology, Ministry of Education of China, Institute of Modern Optics, Nankai University, Tianjin, 300071, China | | | Hua Bai | School of Electronics and Information Engineering, Tianjin Polytechnic University, Tianjin, 300387, China | | | Lie Lin | Key Laboratory of Optical Information Science Technology, Ministry of Education of China, Institute of Modern Optics, Nankai University, Tianjin, 300071, China | linlie@nankai.edu.cn |
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| Abstract: |
| In this paper, we report the discrimination of the viability of human umbilical cord mesenchymal stem cells (hUC-MSCs) with photo-induced delayed luminescence (DL). We measure the DL decay kinetics of hUC-MSCs using an ultraweak luminescence detection system, and find the significant difference in the weight distributions of the decay rate for hUC-MSCs with high and low viabilities. Spectral discrimination of hUC-MSCs with high and low viabilities is thus carried out by comparing the DL kinetics parameters, including the initial intensity, the peak decay rate and the peak weight value. Our results show that the novel optical method for the viability diagnosis of hUC-MSCs has a promising prospect. |
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